Fig. 2: Protein expression and pathway analysis of islets from pregnant women.

a Proteins in islets from pregnant women, volcano plot of proteins expressed in islets from pregnant women compared to exocrine tissue from pregnant women. Significantly upregulated proteins are shown in purple, and downregulated proteins in red. Selected proteins with a log2 fold change > 4 or < −4 are labelled. False discovery rate (FDR) < 0.05 = - log10 FDR > 1.3, S0 = 0.01. b Over-representation analysis of enriched pathways from the KEGG and Reactome databases. Upregulated proteins expressed in islets from pregnant women with a log2 fold change > 1 were analysed using the STRING database for physical protein interactions. FDR < 0.05. c Protein networks clustered by physical interactions between nodes. Networks with three or more proteins present in enriched pathways are shown. Protein labels are displayed, and node colours correspond to the enriched pathways in (b). d Volcano plot illustrating differential protein expression in islets from pregnant women compared to islets in non-pregnant controls. Significantly expressed proteins are shown in pink, and are labelled. FDR < 0.05 = - log10 FDR > 1.3, S0 = 0.01. e Analysis of physical protein network interactions of differentially expressed proteins in islets from pregnant women analysed using the STRING database showing no interactions. FDR < 0.05.