Fig. 4: Solution structure of MIFs in the presence of zinc ions. | Nature Communications

Fig. 4: Solution structure of MIFs in the presence of zinc ions.

From: A cytokine-based designer enzyme with an abiological multinuclear metal center exhibits intrinsic and extrinsic catalysis

Fig. 4: Solution structure of MIFs in the presence of zinc ions.

a DLS measurements of wtMIF and the MIF(Y100H) variant. Experimental conditions: 100 μM MIF (monomer) and 100 μM ZnCl2 in 10 mM MOPS buffer (pH = 7.9). b Binding isotherm of wtMIF and MIF(Y100H) as a function of the monomer protein to zinc ion concentration ratio. The fitting curve is displayed as a dashed black line. Experimental conditions: 150 μM MIF (monomer) was titrated with 1.7 mM ZnCl2 in 10 mM MOPS buffer (pH = 7.9). Inset: thermodynamic parameters for the binding of Zn to MIF(Y100H).

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