Fig. 4: Effect of CSFCM on the migration and proliferation of skin cells. | Nature Communications

Fig. 4: Effect of CSFCM on the migration and proliferation of skin cells.

From: Stem cell secretome armed magneto-actuated micromotors as spatio-temporal manipulators for wound healing acceleration

Fig. 4: Effect of CSFCM on the migration and proliferation of skin cells.The alternative text for this image may have been generated using AI.

Representative cells migration images of HaCaT cells (A) and NIH/3T3 cells (B) 24, 48, 72 h after CSFCM treatment. Quantitative relative scratch area of HaCaT cells (C) and NIH/3T3 cells (D). n = 4 independent samples. Treatment with basic medium was used as the Control. In Fig. 4C, ***p < 0.001, ****p < 0.0001, for the data in 24 h, the exact P value between CSFCM and CM group was 0.0003, and exact p value between CSFCM and CSF group was 0.0002; for the data in 72 h, the exact p value between CSFCM and CM group was 0.0001, and exact P value between CSFCM and CSF group was 0.0002. In Fig. 4D, **p < 0.01, ***p < 0.001, ****p < 0.0001, for the data in 24 h, the exact P value between CSFCM and Control group was 0.0014, and exact P value between CSFCM and CM group was 0.0053; for the data in 48 h, the exact P value between CSFCM and CM group was 0.0002, and exact P value between CSFCM and CSF group was 0.0066. The proliferation of HaCaT cells (E) and NIH/3T3 cells (F) quantified by CCK-8 assay, which demonstrated increased proliferation in the CSFCM-treated group compared to the NC group. n = 4 independent samples. Negative Control (NC): basic DMEM medium with no FBS; Positive Control (PC): basic DMEM medium with 10% FBS. In Fig. 4E, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, for the data in 24 h, the exact p value between CSFCM and NC group was 0.01, the exact P value between CSFCM and CM group was 0.0007, and exact P value between CSFCM and CSF group was 0.0005; for the data in 48 h, the exact P value between CSFCM and CSF group was 0.0001; for the data in 72 h, the exact P value between CSFCM and NC group was 0.0014, the exact P value between CSFCM and CM group was 0.0001, and exact p value between CSFCM and CSF group was 0.0489. In Fig. 4F, *p < 0.05, **p < 0.01, for the data in 24 h, the exact P value between CSFCM and NC group was 0.013, the exact P value between CSFCM and CM group was 0.0171, and exact P value between CSFCM and CSF group was 0.0056; for the data in 48 h, the exact P value between CSFCM and NC group was 0.0388, the exact P value between CSFCM and CM group was 0.0051, and exact P value between CSFCM and CSF group was 0.0048; for the data in 72 h, the exact P value between CSFCM and NC group was 0.0236, the exact P value between CSFCM and CM group was 0.0446, and exact P value between CSFCM and CSF group was 0.0364. Data in C-F were presented as mean ± S.D. p values were analyzed by one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

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