Fig. 5: Temporal dynamics of mesenchymal and osteochondral cell populations.
From: Single-cell expression profiling of bat wing development
a Single-cell trajectories of mesenchymal and osteochondral cell populations in developing bat forelimbs, with arrows indicating predicted developmental paths. b Developmental dynamics of mesenchymal and osteochondral cell populations in bat forelimbs. Dissimilarity matrix, global alignment, and pseudotime shift from comparative alignment of gene modules along the trajectories of bat forelimbs and hindlimbs for chondrogenesis (c) and Osteogenesis (d). The histograms correspond to the developmental stages of bat limbs. e Immunofluorescent co-staining of COL2A1 and EdU labeling assay in the proximally first phalanx of digit III of bat forelimbs and hindlimbs at CS18 and CS20, with DAPI for nuclei. Scale bar: 100 µm. f Relative ratio of EdU-positive cells to COL2A1-positive cells in developing bat forelimbs and hindlimbs. g Immunofluorescent co-staining of ABI3BP and DAPI in the proximally first phalanx of digit III at CS20. Scale bar: 100 µm. h Relative ratio of ABI3BP-positive cells to DAPI-positive cells in developing bat forelimbs and hindlimbs. The number of dots (n = 3) in (f, h) represents the number of independent biological replicates. All data are presented as mean ± SD. P values are from two-tailed Student’s t-tests. Source data are provided as a Source Data file.
