Fig. 2: Altering the nucleotide binding pocket landscape changes the ability of ATP and ATP analogs to induce the closed clamp. | Nature Communications

Fig. 2: Altering the nucleotide binding pocket landscape changes the ability of ATP and ATP analogs to induce the closed clamp.

From: ATP plays a structural role in Hsp90 function

Fig. 2

a Cells expressing Hsp82R32A, Hsp82D40A, Hsp82G118A, Hsp82G121A, or Hsp82G123A were not viable, as evidenced by lack of growth on FOA plates. b Growth rates of cells expressing both wild type Hsp82 and the indicated Hsp82 mutant or empty vector (“ev”) control. Bars are the average growth rates of three biological replicates (each replicate is shown as a circle) and error bars are the standard deviation. Asterisks indicate significant difference from wild type (*** p < 0.001, **** p < 0.0001 by one-way ANOVA). c Cells expressing Hsp82F120A or Hsp82F124A were defective in kinase client chaperoning as evidenced by CFW sensitivity. For (a, c) lines in the images indicate where portions were cropped from different areas of the same plate. d–l The response of the indicated Hsp82 mutant to AMPPNP (red), ATP-γ-S (blue) and ATP (black) in the PET assay. Nucleotides were added to the proteins at T = 10 min.

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