Fig. 6: Cdc37 coordinates Hsp90 conformational dynamics through F120.

a Triple knockout strain MR1154 (relevant genotype shown) can only grow on FOA if a viable combination of Hsp90 and Cdc37 alleles are introduced. Cells transformed with Hsp82 only or Cdc37 only (top two rows) failed to grow on FOA while the same strain co-transformed with both Hsp82 and Cdc37 plasmids (bottom row) grew on FOA. b Functional Hsp90/Cdc37 assay with the indicated Hsp82 mutants and either wild type CDC37 (left column) or cdc37^S14A (right column). Cells expressing Cdc37^S14A and either Hsp82^E33A or Hsp82^F120A were inviable. The images in each panel were cropped from the same plate. c Like in (b) except the viable F120 substitutions in Hsp82 were combined with cdc37^S14A. Only the wild type phenylalanine at position 120 supported normal growth in cells expressing Cdc37^S14A. The images in (a, c) were all cropped from the same plate.