Table 1 Summary of in vivo and in vitro mutational analyses

Mutant	In vivo			In vitro				Ref
	Func^a	Phenotype	DN^b	AMPPNP^c	ATP-γ-S^c	ATP^c	ATPase^d
WT	Yes	No	No	100	100	-	1
E33A	Yes	Slow, CFW^S	No	-	-	80	none^e	^29,30
R32A	No	n/a	No	-	-	-	0.54 ± 0.02	⁴⁷
D40A	No	n/a	No	-	-	-	0.15 ± 0.07	²⁴
K98A	Yes	No	No	100	100	50	0.93 ± 0.03	^27,30
G118A	No	n/a	Yes	> open^f	> open	80	none	²⁴
F120A	Yes	Slow, CFW^S	No	-	100	50	1.29 ± 0.02	^7,8,53
G121A	No	n/a	No	-	-	-	0.03±0.02	²⁴
G123A	No	n/a	Yes	>open	100	10	0.61 ± 0.02	²⁴
F124A	Yes	Slow, CFW^S	No	80	100	100	3.33 ± 0.07	^24,76
A152V	Yes	No	No	100	100	100	1.80 ± 0.16	³⁷
R380A	No	n/a	No	-	-	-	0.53 ± 0.03	^13,15,29
R380K	Yes	No	No	50	100	50	0.98 ± 0.08

^a func function in vivo (support viability).
^b DN dominant negative phenotype.
^c AMPPNP, ATP-γ-S, ATP the approximate level of closed clamp stabilization induced by the nucleotide observed in PET assays, compared to wild type.
^d ATPase rate relative to wild type.
^e none ATP hydrolysis activity not detected.
^f >open more open compared to the apo state of this mutant.

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