Fig. 7: iTRF-induced age mosaic results in alleviated midgut hyperplasia and extended lifespan. | Nature Communications

Fig. 7: iTRF-induced age mosaic results in alleviated midgut hyperplasia and extended lifespan.

From: Age mosaic of gut epithelial cells prevents aging

Fig. 7: iTRF-induced age mosaic results in alleviated midgut hyperplasia and extended lifespan.The alternative text for this image may have been generated using AI.

a Schematic of ad libitum (Ad) and iTRF treatment. b Time courses of statistics of PH3+ cells per midgut in Ad and iTRF flies. The light blue indicates the time window in which the iTRF fasting is in place. n, numbers of midguts. Ad: n = 14 (10 d), 16 (16 d), 16 (20 d), 14 (24 d), 12 (34 d), 12 (47 d), 14 (57 d). iTRF: 12 (10 d), 20 (16 d), 16 (20 d), 12 (24 d), 14 (34 d), 14 (47 d), 14 (57 d). c Upper, design of the puparium H2B-RFP EC labeling and tracing in Ad and iTRF flies. Lower, representative images of the RFP tracing pattern in 18 d, 26 d Ad and iTRF midguts. d Statistics of the ratio of RFP+ ECs to total ECs in selected areas of Ad and iTRF posterior midgut during aging. n, numbers of regions. Ad: n = 25 (14 d), n = 22 (18 d), n = 21 (22 d), n = 22 (26 d); iTRF: n = 17 (14 d), n = 20 (18 d), n = 17 (22 d), n = 20 (26 d). e Statistics of PH3+ cells in Ad midgut in which Atg1 was overexpressed in ECs during the night. attp2: n = 19, Atg1: n = 20. f Schematic of Atg1 or Atg8a knockdown in ECs in 30 °C time windows (the iTRF fasting phase) in Ad or iTRF flies. g, h Statistics of PH3+ cells per midgut in Ad and iTRF flies counted at 24 d (g) or 70 d (h) when Atg1/Atg8a was knocked down in the EC within 30 °C time windows (f), n, numbers of midguts. n (24 d, from left to right) =18, 20, 20, 19, 19, 20; n (70 d, from left to right) =18, 19, 20, 17, 19, 19. Representative images (i) and statistics (j) of Dlg1 staining in Ad and iTRF midgut at 35 d. GFP driven by esg-Gal4 indicates the ISCs/EBs. n, number of ECs measured. 16 (Ad) and 17 (iTRF) midguts are examined. k Upper: schematic of the prevention of age mosaic formation by blocking ISC divisions (esgts>Cdk2 RNAi) in Ad and iTRF flies. Lower: statistics of the PH3+ cells per midgut in control (attp2) and age mosaic prevention (Cdk2 RNAi) files on Ad and iTRF treatment at 55 d. n, numbers of midguts. n = 13, 16, 15, 15 (from left to right). l Survival analysis of control (attp2) and age mosaic prevention (Cdk2 RNAi) files on Ad and iTRF treatment. Data are mean ± SEM. Significance was determined using two-tailed unpaired t test. Scale bars, 10 μm (I) or 20 μm (c). Source data are provided as a Source Data file.

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