Fig. 4: GH9C2 is required for cellulose degradation during rhizobial release.
From: A legume cellulase required for rhizobial infection and colonization in root nodule symbiosis
a, b Typical cell of WT (a) and gh9c2-1 (b) after rhizobia release from infection threads. c, d Quantification of enlarged rhizobia release foci (c) and presence of S4B staining in rhizobial release foci (d) in WT and gh9c2-1 nodule cells. N = 180 (WT) and 209 (gh9c2-1). e–l S4B staining at different stages of rhizobial release in WT (e-h) and gh9c2-1 (i-l) nodule cells. Rhizobia were stained with SYTO13. Arrows indicate S4B staining in IT wall. Arrowheads indicate S4B staining in rhizobial release foci (f, k) and cluster of released rhizobia (l). Double arrowheads indicate excessive accumulation of S4B staining in gh9c2-1 (j). rrf, rhizobial release foci. For S4B staining assay, rhizobial release phenotyping was collected from three independent experiments, with at least 16 nodules in each experiment. Scale bars, 5 µm (a, b) and 10 µm (e–l).
