Fig. 5: Impairing the Erg11-Ncp1 interaction leads to the release of Ca²⁺ from the ER and accumulation in the mitochondria.

a Representative images depicting the localization of Ca²⁺ localization (green) in relation to ER membranes (red) were obtained from ERG11/erg11Δ and erg11^m/erg11Δ mutants. Scale bar = 5 µm. b The integrated green fluorescence intensity (post-merge) was quantitatively analyzed between the ERG11/erg11Δ and erg11^m/erg11Δ mutants using Image J software. The integrated fluorescence intensity was calculated as the product of the fluorescence density and the fluorescence area. c An assay was conducted to observe for Ca²⁺ activation (red) in the mitochondria using confocal microscopy. In the diagram provided, the large black ellipse symbolizes the C. albicans cell, the small orange ellipse represents the mitochondria, and the red dot signifies Ca²⁺. Scale bar = 5 µm. d The mitochondrial Ca²⁺ levels in confocal microscopy images were quantitatively analyzed between the ERG11/erg11Δ and erg11^m/erg11Δ mutants using Image J software. The integrated fluorescence intensity was calculated as the product of the fluorescence density and the fluorescence area. Three biological replicates were performed, and a representative image is shown (a, c). Violin plots display the distribution density (width of the violin shape), median (black points), interquartile range (white points), and range (minimum and maximum values) (b, d). Two-tailed unpaired t-test (b, d). Source data are provided as a Source Data file.