Fig. 6: dMterf4 RNAi-initiated downregulation of LManVI aggravates dMterf4 RNAi-mediated mitochondrial defect by PGC-1. | Nature Communications

Fig. 6: dMterf4 RNAi-initiated downregulation of LManVI aggravates dMterf4 RNAi-mediated mitochondrial defect by PGC-1.

From: Impaired mitochondria-initiated crosstalk with lysosomes reciprocally aggravates mitochondrial defect through LManVI

Fig. 6

a In dMterf4 RNAi background, knockdown of Med8 and Tfb4 restored the transcription levels of Acon, Tim13, ttm3, PGC-1, mtTFB2, and mtTFA. b, b’ Overexpression of HA-LManVI in dMterf4 RNAi background increased the transcription of Tim13, ttm3, PGC-1, and mtTFB2 except for Acon and mtTFA. c The epitasis experiments showed that both PGC-1 and mtTFB2 worked at the downstream of LManVI. d, e Overexpression of PGC-1-HA in dMterf4 RNAi background increased transcription levels of Tim13, ttm3, and mtTFB2 (d) and mitochondria-encoded genes ND1, ND3, ND4, ND5, CoI, CoIII, ATPase6, and Cytb (e). f–h Knockdown of PGC-1 in dMterf4 RNAi background reversed HA-LManVI overexpression-mediated rescue effect of the abnormal wing phenotypes (f), and the transcriptional upregulation of Tim13, ttm3, and mtTFB2 (g) and mitochondria-encoded genes ND1, ND3, ND4, ND5, CoI, CoIII, ATPase6, and Cytb (h). i The epitasis experiments showed that mtTFB2 worked at the downstream of PGC-1. j Overexpression of mtTFB2-HA in dMterf4 RNAi background increased the transcription of ND1, ND3, ND4, ND5, CoI, CoIII, ATPase6 and Cytb. k Overexpression of HA-EC3 upregulated the transcription levels of Tim13, ttm3, and mtTFB2 in dMterf4 knockdown flies. l, m Knockdown of EC3 inhibited PGC-1-HA overexpression-mediated rescue effect of abnormal wings (l) and reversed the PGC-1-HA-mediated increased expression of Tim13, ttm3, and mtTFB2 (m) in dMterf4 RNAi background. All data were performed with three biological replicates (n = 3). For sample sizes: n = 10 fly thoraxes (a, b, b’, d, e, g, h, j, k, and m), 100 fly pupae (c), or 100 flies (f, i, and l) were used in each replicate. The error bars are mean ± SD. For the statistics, one-way ANOVA with Tukey’s multiple comparisons test was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns means no significant difference. Data points indicate biological replicates. Source data were provided as a Source Data file.

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