Fig. 8: The LManVI-mediated communication between mitochondria and lysosomes may be conserved in mitochondria-damaged mammalian cells.

a In knockdown of MTERF4 background, knockdown of mMed8 and mGtf2h3 partially restored the decreased MAN2B1 mRNA level in C2C12 cells. b Knockdown of MTERF4 reduced, while overexpression of HA-MAN2B1 partially restored mature CathepsinD protein level in C2C12 cells (Actin: loading control, representative pictures were shown). c Protein levels of (b) were quantified with ImageJ. d, e Knockdown of MAN2B1 in C2C12 cells downregulated mitochondria-related genes Acon, PGC-1α, and Timm50 mRNA levels (d) and the ATP levels (e) (The plotted value is the mean of three biological replicates). f In C2C12 cells treated with Rot, MAN2B1 mRNA level decreased, knockdown of mMed8 and mGtf2h3 in this background restored its mRNA level (The plotted value is the mean of three biological replicates). g In C2C12 cells treated with Rot, mature CathepsinD protein level decreased, overexpression of HA-MAN2B1 in this context partially restored mature CathepsinD protein level (Actin: loading control, representative pictures were shown). h Protein levels of (g) were quantified with ImageJ. i, j In Rot-treated C2C12 cells, knockdown of MAN2B1 resulted in lower ATP levels (i), overexpression of HA-MAN2B1 partially restored the ATP levels (j). The error bars are mean ± SD, All data were performed with three biological replicates (n = 3). Data points indicate biological replicates. For the statistics, two-tailed unpaired t-test (d and e), or one-way ANOVA with Tukey’s multiple comparisons test (a, c, f, h, i, and j) was used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns means no significant difference. Source data were provided as a Source Data file.