Fig. 2: The N-terminal domains of D10 and D2 form amyloid fibrils. | Nature Communications

Fig. 2: The N-terminal domains of D10 and D2 form amyloid fibrils.

From: Amyloid fibril structures link CHCHD10 and CHCHD2 to neurodegeneration

Fig. 2: The N-terminal domains of D10 and D2 form amyloid fibrils.The alternative text for this image may have been generated using AI.

a ThT monitored aggregation of purified recombinant D10-NT (black) and D2-NT (green). b Time to reach half maximal ThT fluorescence (T50) for D10-NT and D2-NT assembly (mean ± SD, n = 3 biological replicates, unpaired two-tailed paramettric Student’s t-test; *p = 0.031). c TEM micrograph of D10-NT agitated at ca. 20 µM concentration for 24 h at 1000 RPM. Similar results were obtained for multiple (>2) independent samples/experiments. d TEM micrograph of D2-NT agitated at ca. 80 µM concentration for 192 h at 1200 RPM. Results were obtained from a single sample/experiment but are corroborated by cryo-EM images of independent samples. e Skin fibroblasts from p.R15L D10 patients and controls immunostained for D10 (cyan) and co-stained with ThioS (magenta), showing colocalization (arrows). Scale bars: 50 μm. Similar results were obtained from a second independent sample/experiment. Source data for a and b are provided as a Source Data file.

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