Fig. 1: Global RNA-Protein Interactome purification (GRPIp) screening identifies RRP1 in IL-1-stimulated primary macrophages.
From: The RNA-binding protein RRP1 brakes macrophage one-carbon metabolism to suppress autoinflammation
a Schematic of the GRPIp method (See Methods). b Distribution of RNA-RBP complex extracts after proteinase K or RNase A digestion analyzed by agarose gel electrophoresis. Line 1: the purified total RNA from the BMDMs. Line 2 to Line 4: RNA-RBP extracts purified from the interphase. (QC test ① mentioned in the Schematic). c Distribution of samples after RNase digestion, trypsin/Lys-C fragmentation by HPLC analysis (QC test ② mentioned in the Schematic). GO analysis (d) and Venn diagram (e) of potential RBPs identified in BMDMs stimulated by IL-1β for 0 h, 1 h and 12 h. f, g RT-qPCR detection of Il6 and Il-1b mRNA levels in BMDMs with siRNA-mediated RRP1 silence followed by IL-1β stimulation for the indicated hours. h ELISA of IL-6 levels in the supernatant of BMDMs with siRNA-mediated RRP1 silence followed by IL-1β stimulation for the indicated hours. Data are representative of three independent experiments (b, c). Data are of technical replicates from three independent experiments (n = 3) (d, e) with hypergeometric test (one-tailed) (d). Data are presented as means ± SD of (f, g, n = 3; h, n = 4) biologically replicates from three independent experiments with student’s t test (two-tailed unpaired). Source data are provided as a Source Data file.
