Fig. 6: Chromatin three-dimensional structural characteristics in CTCs during mitosis and interphase.

a, b Interaction heatmaps of chromatin within CTCs at 1 Mb, 500 Kb, and 100 Kb resolutions, during the mitotic and interphase stages, respectively. c, d Saddle plots of genome-wide interaction maps for mitotic and interphase CTCs. The saddle plots were constructed by sorting the interaction matrix according to the eigenvector values from the lowest (B) to the highest (A) values. The sorted maps were then normalized to their expected interaction frequencies. The upper left of the interaction matrix indicates the strongest B-B interactions, the lower right shows the strongest A-A interactions, and the upper right and lower left quadrants represent B-A and A-B interactions, respectively. The saddle plots were quantified by taking the top 20% of B-B interactions and the top 20% of A-A interactions, normalized by the bottom 20% of A-B interactions. e Distribution of A/B compartments in mitotic and interphase CTCs, with the y-axis representing eigenvectors. Values greater than zero denote the A compartment and values less than zero denote the B compartment. The resolution on the x-axis is 100 kb. Simulated three-dimensional chromatin structures, with (f) and (g) representing the nuclear structure of a cell in mitosis, including the spatial organization of individual chromosomes, and (h) and (i) depicting the chromatin spatial structure during interphase. Source data are provided as a Source Data file.