Fig. 4: pre-tRNATyr 1-2 is modified at G34 with galQ before splicing. | Nature Communications

Fig. 4: pre-tRNATyr 1-2 is modified at G34 with galQ before splicing.

From: Queuosine is incorporated into precursor tRNA before splicing

Fig. 4: pre-tRNATyr 1-2 is modified at G34 with galQ before splicing.The alternative text for this image may have been generated using AI.

a The presence of galactose modification on Q34 can eliminate its capacity to interact with boronic acid derivative in the ABP-PAGE. β1-3,4 Galactosidase (β-GAL) is used to hydrolyze terminal β-galactose residues from the precursor and mature tRNATyr. Blue circles indicate the secondary amine protonated and reacting in AD Northern blot. Red rectangle indicates the cis-diol group reacting in APB Northern. b Both pre-tRNATyr1-4 and 1-2 are shifted in wild type mESCs using AD Northern indicating the presence of a Q derivative modification. c Digestion of galQ using β1-3,4 Galactosidase (β-GAL) revealed the Q derivative modification on mature m_tRNATyr and m_pre-tRNATyr1-2 in mESCs and the mouse brain using gdAPB Northern blot.

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