Fig. 5: Evolutionary analysis of the pre-tRNATyr modification at position 34 using Northern blot.
From: Queuosine is incorporated into precursor tRNA before splicing
a galQ modification is detected on mature h_tRNATyr and on h_pre-tRNATyr 8-1 and 9-1 in human HeLa cells grown in medium ± q using gdAPB Northern blot. b D. melanogaster is missing QTGAL enzyme and Q modification is detected on d_pre-tRNATyr 1-4 in S2 cells grown in medium ± q using APB Northern blot. c Q modification is detected on mature c_tRNATyr and on c_pre-tRNATyr 2-8 in N2 wild type strain of C. elegans grown in peptone rich medium (LB) versus NGM plates (N) using gdAPB Northern blot. Red arrows indicate Q, black arrows indicate unmodified or unresolved galQ.
