Fig. 4: Specific RAP250 repression in BAT phenocopies many of the metabolic alterations of RAP250+/− mice, and thermoneutrality normalizes the metabolic alterations of RAP250+/− mice.
From: Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue
Representative image for RAP250 protein expression (A) and quantification (B) in BAT extracts from 32-week-old male mice 24 weeks after injection with Scr or ShRAP250 AAVs into BAT. Data were normalized to Vinculin (n = 5 for Scr and ShRAP250). Body weight (C) and total fat mass (D) curves after AAV administration into BAT (Scr, n = 5, black symbols; ShRAP250 n = 4, green symbols). Glucose oxidation (E) and RER (ratio CO2/O2) (F) over 24 h in 28-week-old male mice injected with Scr or ShRAP250 AAVs into BAT and on a normal diet. Gray phase corresponds to dark phase (n = 4 for WT; n = 4 for RAP250+/−). G UCP1 expression quantification in BAT extracts. Data were normalized to Vinculin (n = 5 for Scr and ShRAP250). H Plasma leptin from 16 h fasted mice (n = 5 for Scr and ShRAP250). Body weight curves from 8- to 22-week-old male mice subjected to thermoneutrality and on a normal chow diet (TN-ND) (I) or a high-fat diet (TN-HFD) (J). Data as grams of body weight (WT, n = 7; RAP250+/− n = 8 for TN-ND and WT, n = 4 and RAP250+/− n = 6 for TN-HFD). Black symbols for WT mice and red symbol for RAP250+/−. K Total fat mass from 22-week-old mice under TN-ND and from 20-week-old mice under TN-HFD. Data as grams of FAT (WT, n = 5, RAP250+/− n = 6 for TN-ND and WT, n = 3, RAP250+/− n = 5 for TN-HFD). L Brown adipose tissue weight. Data are expressed as percentage of tissue weight with respect total body weight (TN-ND WT, n = 5, RAP250+/− n = 6; TN-HFD WT n = 3, and RAP250+/− n = 5). Paraffin sections of PAT stained with Hematoxylin/Eosin for TN-ND (M) and TN-HFD (N) (scale bar=100 µm). O PAT adipocyte cell size of TN-ND (22-week-old) and TN-HFD (20-week-old) (WT TN-ND n = 5, RAP250 TN-ND n = 6, WT TN-HFD n = 3 and RAP250 TN-HFD n = 4, from 8−10 images per mouse). Data are MEAN+/− SEM. Statistical differences according to a two-sided Student´s t test (B and G) and Ordinary two-way ANOVA (C, D, E, F and J) and two-way ANOVA followed by Sidak’s multiple comparison test (K, L and O). A Created in BioRender. Bausa, O. (2025) https://BioRender.com/rdnlgxb. Source data are provided as a Source Data file.
