Fig. 5: Activity is increased across whole brain, including sleep-promoting circuits, after sleep restriction. | Nature Communications

Fig. 5: Activity is increased across whole brain, including sleep-promoting circuits, after sleep restriction.

From: Sleep drive, not total sleep amount, increases seizure risk

Fig. 5: Activity is increased across whole brain, including sleep-promoting circuits, after sleep restriction.

Brains dissected from nsyb-Gal4>CaLexA flies treated with vehicle or caffeine. a Representative images in the anterior, medial, and posterior brain revealing the mushroom body (MB), ellipsoid body (EB), and dorsal fan-shaped body (dFSB). White lines outline the region of interest used for subregion quantification. Images are pseudocolored with the “fire” lookup table with warmer colors indicating increased intensity. b–e Sleep restriction with caffeine in nsyb-Gal4>CaLexA flies leads to increased whole brain, MB, EB, and dFSB CaLexA (GFP:RFP) signal. n = 10-21 flies/condition. Two-group two-tailed t-test. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.

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