Fig. 5: Tissue penetration and clearance pathways. | Nature Communications

Fig. 5: Tissue penetration and clearance pathways.

From: Unimolecular near-infrared chemiluminescent reporter for cascaded multiplex imaging of ischemia-reperfusion injury in the liver-kidney axis

Fig. 5

a Schematic illustration of tissue-penetration study. b, c Chemical structures and chemiluminescence spectra of BD-OTf, HCL1-OTf and HCL5-OTf. d Representative chemiluminescence and fluorescence images of BD-OTf, HCL1-OTf and HCL5-OTf (40 µM) solutions upon overlaying chicken tissues with the desired thickness on top of the samples. e, f SBRs for chemiluminescence and fluorescence imaging of BD-OTf, HCL1-OTf and HCL5-OTf as a function of chicken tissue depth in panel d. (n = 3, mean ± s.d.). g Schematic illustration of renal and hepatobiliary clearance pathways in living mice. h Blood concentration (% ID g−1) decay of CAR, KIR and LIR after i.v. injection into living mice. (n = 3, mean ± s.d.). i The total renal and fecal clearance efficiency of CAR, KIR and LIR after 7 days of injection. (n = 3, mean ± s.d.). j Fluorescence images of the abdominal cavity of mice after 1 h injection of CAR, LIR, FL2R, PBS, KIR and FL1R (2.5 μmol kg−1 body weight). Bladder (Bl), kidneys (Ki), liver (Li), muscle (Mu), spleen (Sp). k NIR-II fluorescence intensity of liver from mice after 1 h injection of CAR, LIR and FL2R. (n = 3, mean ± s.d.; a.u. means arbitrary units) l NIR-I fluorescence intensity of kidneys after 1 h injection of PBS, KIR and FL1R. (n = 3, mean ± s.d.). Source data are provided as a Source Data file.

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