Fig. 2: Comparison of DENV-2 titers and relative Wolbachia densities at 7 dpi by feeding and dissemination status.

a Body DENV-2 titers in single- and double-fed wildtype mosquitoes lacking Wolbachia (WT), wAlbB, and wMelM mosquitoes. WT SF n = 34, WT DF n = 42, wAlbB SF n = 46, wAlbB DF n = 35, wMelM SF n = 11, wMelM DF n = 27. WT single-fed vs double-fed p = 0.0001 and wAlbB single-fed vs double-fed p < 0.0001. b Relative body Wolbachia density in single- and double-fed wAlbB and wMelM mosquitoes. Numbers tested are as follows: wAlbB SF n = 74, wAlbB DF n = 61, wMelM SF n = 119, and wMelM DF n = 148. wAlbB single-fed vs double-fed p = 0.0027. c Body DENV-2 titers in wAlbB mosquitoes by dissemination status and feeding status. Total Dis n = 46 and Not Dis n = 35. Dis SF n = 18, Dis DF n = 28, Not Dis SF n = 28, and Not Dis DF n = 7. Dis vs Not Dis p < 0.0001. d Relative body Wolbachia densities in wAlbB mosquitoes by infection, dissemination, and feeding status. Total Dis n = 46, Not Dis n = 35, and Not Inf n = 54. Dis SF n = 18, Dis DF n = 28, Not Dis SF n = 28, Not Dis DF n = 7, Not Inf SF n = 28, and Not Inf DF n = 26. Comparisons were made using two-tailed Mann-Whitney U tests (a–c) or a Kruskal-Wallis test with Dunn’s multiple comparisons (d). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p < 0.0001. Blue = single-fed, red = double-fed. GE = genome equivalents, Dis = mosquitoes with a disseminated infection (i.e., dengue infected bodies and legs), Not Dis = mosquitoes with a non-disseminated infection (i.e., dengue infected bodies, but not legs), Not Inf = mosquitoes that were exposed but are not infected with DENV-2. Lines indicate median with 95% confidence interval. Data was collected across 4 replicates for WT and wAlbB groups and 5 replicates for wMelM groups. Source data are provided as a Source Data file.