Fig. 5: COUP-TFII-driven capillary-to-venule reprogramming enhances T cell recruitment from circulation.

A Schematics and quantification of lymph node T cell homing assay in PyMT-B6 tumors. (n = 8 biological replicates/group). Representative of more than three independent experiments. B Schematics, representative flow cytometry plots, and quantification of OT1 T cell homing to orthotopic PyMT-OVA tumors. (n = 3 biological replicates/group). Representative of three independent experiments. C Flow cytometric analyses of bone marrow cell homing to orthotopic PyMT tumors. (n = 8 biological replicates/group). Representative of more than three independent experiments. Quantification in (B, C) indicates the frequency of homed donor-derived leukocytes as % of total cells dissociated from the tumor. D Gating strategy for circulating leukocytes in the blood. E Frequencies of circulating leukocyte subsets in the blood of iCoup and control mice, quantified as the percentage among circulating CD45+ cells. (n = 4 biological replicates/group). F–I Frequencies of indicated T cell populations in the tumor-draining lymph nodes (dLNs). (control: n = 10 biological replicates, iCoup: n = 12 biological replicates). J–Q Representative gating strategy and flow cytometric quantifications of indicated T cell subsets in the dLNs. (control: n = 10 biological replicates, iCoup n = 12 biological replicates). Groups were compared by an unpaired two-tailed Student’s t-test, ns denotes not significant. Error bars indicate s.e.m.