Fig. 6: LFA-1 integrin controls the accumulation of CD8⁺CD103^– cells in the brains of 3xTg- AD mice.

a Flow cytometry showing the percentage of LFA-1⁺ CD8⁺ T cells (left) and corresponding MFIs (right) in the blood of WT (n = 6) and 3xTg-AD (n = 6) mice. Data are means ± SD, with P-values based on two-tailed Student’s t-test. b Flow cytometry showing the percentage of CD45+ leukocytes among live cells. Data are means ± SEM, with P-values based on two-tailed Student’s t-test. c Flow cytometry showing the percentage of CD103^– (left) and CD103⁺ (right) CD8⁺ Trm cells in the brains of 3xTg-AD (n = 6) and 3xTg-AD/Itgal^–/– (n = 6) mice. Data are means ± SEM, with P-values based on two-tailed Student’s t-test. d–f Representative immunohistochemical staining of the hippocampus in 3xTg-AD (n = 3) and 3xTg-AD/Itgal^–/– (n = 3) mice, showing the Aβ load (c), and the levels of hyperphosphorylated (d) and total (e) tau protein. Scale bar = 20 µm. Data are means ± SEM, with P-values based on two-tailed Student’s t-test. g UMAP plot showing immune cell populations in human blood (n = 3 AD patients and n = 2 negative controls, NCs) by scRNAseq. h Normalized expression of CD3G, CD3D and CD3E on a UMAP plot. Transcript levels are color- coded i CD3⁺ T cell cluster subsets: UMAP plot showing CD8⁺ and CD4⁺ T cell subpopulations in human blood (n = 3 AD patients and n = 2 NCs). j Normalized expression of CD8A, CD8B and CD4 on a UMAP plot. Transcript levels are color- coded. k CD8⁺ T cell cluster subsets: UMAP plot showing early active, Tem, and naïve-like CD8⁺ T cells in human blood (n = 3 AD patients and n = 2 NCs). l Radar plot showing AUCell score using known genes. m Heat map reporting the expression values (mean calculated using the Hurdle model) of ITGAL in AD patients (n = 3) and NCs (n = 2). Transcript levels are color-coded.