Fig. 9: GrK induces functional alterations in human neuronal cells via PAR-1. | Nature Communications

Fig. 9: GrK induces functional alterations in human neuronal cells via PAR-1.

From: CD103–CD8+ T cells promote neurotoxic inflammation in Alzheimer’s disease via granzyme K–PAR-1 signaling

Fig. 9

a Immunofluorescence staining for βIII-tubulin, MAP-2, pNF-H, Nestin and PAR-1 in undifferentiated and differentiated SH-SY5Y human neuroblastoma cells. b Intracellular Ca2+ release in differentiated SH-SY5Y cells cultured in the presence of 150 nM GrK, or 150 nM GrK and 100 nM SCH79797. The last time point is shown in the bottom panel. Ctrl– = neurons alone. Ctrl+ = ionomycin-stimulated neurons (10 µM). Data are means ± SEM from three independent experiments. P-values based on two-way ANOVA-multiple comparisons. c Representative images of differentiated SH-SY5Y cells at different time points cultured without GrK (Ctrl–), in the presence of 150 nM active GrK, or in the presence of 150 nM active GrK plus 100 nM SCH79797. The red signal shows intracellular Ca2+ release. Scale bar = 20 µm.

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