Fig. 3: Modeling and validation of Izumo1:Juno forming a catch bond.
From: Multi-state catch bond formed in the Izumo1:Juno complex that initiates human fertilization
a Proposed three-state kinetic model for unbinding of Izumo1:Juno with reversible transitions between multiple structural states. b Monte Carlo simulated force clamp AFM-SMFS for the Izumo1:Juno complex. Colored dots represent mean bond lifetime for each unbinding pathway at each setpoint with intensity representing the relative frequency of an unbinding pathway. Colored lines represent lifetimes calculated directly from BE-derived kinetic parameters (Supplementary Table 1, Eq. (11)). Black line represents the mean bond lifetime across all three pathways. c Schematic showing protein constructs and surface immobilization for force clamp AFM-SMFS. d Experimental bond lifetimes for Izumo1:Juno from force clamp AFM-SMFS (representative of the consensus across the three biological replicates performed). Colored dots represent individual bond lifetime measurements performed. Black dots represent median bond lifetime (error bars ± MAD) across 10 pN bins (n = 18, 18, 33, 53, 44, 38, 9). Shared letters indicate the absence of statistically significant differences (p > 0.05) from Kruskal–Wallis tests followed by Dunn’s multiple-comparison tests (Šidák-adjusted). Exact P-values are provided in the Source Data file. Full statistical details are provided in the “Methods”.
