Fig. 4: Juno H177 mediates the force-initiated transition to higher order unbinding pathways in Izumo1:Juno.
From: Multi-state catch bond formed in the Izumo1:Juno complex that initiates human fertilization
a Exemplary snapshot of stable long-lasting Cluster 3 interactions mediated by Juno H177. b Exemplary snapshot of terminal complex-prolonging interactions mediated by Izumo1 K48 and Juno H177. c Exemplary snapshot of transient Cluster 3 interactions formed in the Izumo1:JunoH177Q infertility mutant. d Exemplary snapshot of terminal interactions, the last ones to break prior rupture, that are absent in Izumo1:JunoH177Q. e Observed decreases in contact area as derived from SMD between Izumo1 and JunoH177Q in unloaded (t = 0 ns) and loaded (t = 2 ns) complexes for the two-state (2-S) and three-state (3-S) unbinding pathways. f Peak forces extracted from two-state and three-state SMD simulations of Izumo1:JunoH177Q complexes (median ± MAD). g Two-state and three-state unbinding pathway distribution for Izumo1:H177Q across 50 SMD trajectories. For (e–g), n = 21 and n = 29 for 2-S and 3-S, respectively. Boxplots show the median, interquartile range (IQR), and whiskers extending to 1.5 times the IQR. Points beyond the whiskers are outliers.
