Fig. 5: Constant speed and force clamp AFM-SMFS reveals the loss of mechanostability in Izumo1:JunoH177Q.
From: Multi-state catch bond formed in the Izumo1:Juno complex that initiates human fertilization
a Rupture force vs. loading rate plot for Izumo1:JunoH177Q (median, error bars ± MAD). Dashed lines show the loading rate vs. rupture force behavior predicted by DHS model fitting (Eqs. (6) and (7), Supplementary Table 1). b Izumo1:JunoH177Q rupture force histograms (bins = 20 pN). Data in (a and b) represent four pooled independent experiments that were divided based on unbinding pathway for DHS model fitting (P0: n = 325, 145, 113, 266. P1: n = 113, 62, 116, 188. P2: n = 63, 73, 4, 39). For (c), significance (*p < 0.05) was determined using Kurskal–Wallis tests followed by pairwise Wilcoxon rank-sum tests. c Bond lifetimes for Izumo1:JunoH177Q from force clamp AFM-SMFS (representative of the consensus across the three biological replicates performed). Colored dots represent individual bond lifetime measurements. Black dots represent median bond lifetime (error bars ± MAD) across 10 pN bins (n = 1, 7, 10, 11, 17, 11, 9). Shared letters indicate the absence of statistically significant differences (p > 0.05) using Kruskal–Wallis tests followed by Dunn’s multiple-comparison tests (Šidák-adjusted). Exact P-values are provided in the Source Data file. Full statistical details are provided in the “Methods”.
