Fig. 2: Characterization of the translocation of q-arm of chromosome 10 on q-arm of chromosome X.
From: The reference genome of the human diploid cell line RPE-1
a Bandage visualization showing how chromosome 10 and chromosome X are joined in a single connected component in the assembly graph produced by Verkko v2.0, as a result of the RPE-1 specific t(X;10)(Xq28;10q21.2) rearrangement. b Representative G-banded karyogram of RPE-1 chromosomes obtained using GIEMSA staining. Scale bar: 10 μm. The normal and rearranged X chromosomes are shown (zoom in). This experiment was independently repeated ten times with similar results. c Hi-C contact maps, visualized using PretextView, showing the two haplotypes at chromosome-level resolution. d Zoom in of the Hi-C contact map of the diploid genome highlighting chromatin interaction between chromosome 10 and X due to the t(X;10)(Xq28;10q21.2) rearrangement. e Schematic representation of the multi-step approach followed to curate the breakpoint between chromosome 10 and X at base-pair resolution.
