Fig. 3: Time to detection of optimized multiplexed HPV16/gDNA reaction on Bio-Rad thermocycler and benchtop T8-ISO. | Nature Communications

Fig. 3: Time to detection of optimized multiplexed HPV16/gDNA reaction on Bio-Rad thermocycler and benchtop T8-ISO.

From: One-hour extraction-free loop-mediated isothermal amplification HPV DNA assay for point-of-care testing in Maputo, Mozambique

Fig. 3

A range of concentrations of SiHa (HPV16) DNA and gDNA were used as targets to evaluate the limit of detection of the reaction. The reaction was performed on the Bio-Rad (A, B) for probit analysis (n = 9) and was read on the A FAM channel for HPV16 detection and B ROX channel for gDNA detection. The reaction was also performed on the benchtop T8-ISO (C, D), also read on the C FAM channel for HPV16 detection and D ROX channel for gDNA detection (n = 3). Bar graphs represent mean time to detection and error bars represent standard deviation. Number of replicates that amplified out of total replicates performed are specified on the bar graphs. Source data are provided as a Source Data file.

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