Fig. 2: RcpC33-303 forms a dodecameric oligomeric assembly that interacts with RcpA.
From: The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension
A SEC-MALS analysis of RcpC33-303. The light scattering trace is in cyan, and the molecular weight of the corresponding peak is in red, revealing a molecular weight of ~ 365 kDa. B Negative-stain TEM micrograph of purified RcpC33-303, revealing that it forms ring-like oligomers. Negative stain micrograph is representative of three independent repeats. C Gel filtration UV traces for RcpC33-303 (Cyan), RcpAN (Green), or the RcpC33-303-RcpAN complex (Orange). The corresponding SDS-PAGE gels are shown below. Analytical SEC and subsequent SDS PAGE analysis are representative of three independent repeats. D SDS PAGE analysis of glutaraldehyde crosslinking of the RcpC33-303-RcpAN complex. The band for RcpAN is absent in the presence of crosslinker, where additional bands of higher molecular weights appearing, corresponding to the crosslinked RcpC33-303-RcpAN complex. Crosslinking experiment shown here is representative of three independent repeats. E SEC-MALS analysis of the RcpC33-303-RcpAN complex, colored as in (A). The molecular weight of the complex is ~412 kDa.
