Fig. 5: Evaluation of the sensitivity of vPro-MS for the identification of SARS-CoV-2 in nasal swabs.

The sensitivity of vPro-MS for the identification of SARS-CoV-2 was evaluated in 66 respiratory swab samples. The panel included 8 negative samples and 58 SARS-CoV-2 positive samples covering a Ct range of 18–35 of three different variants (alpha, delta, omicron). The quantitative values for proteomics were calculated as the sum of the three most intense peptides (Top3) and compared to qPCR (Ct). Results were compared when using the vPro peptide library (A) and a library constructed from the analysis of synthetic SARS-CoV-2 peptides (B). The limits of detection are displayed at 95% confidence.