Table 1 Mutations acquired after serial passaging chimeric HS-binding mutants

From: Three positively charged binding sites on the eastern equine encephalitis virus E2 glycoprotein coordinate heparan sulfate- and protein receptor-dependent infection

SINV/EEEV eGFP TaV

E2 amino acid position

8

71

74

77

84

119

147

156

157

206

244

WT

Q

K

K

K

R

R

E

K

R

E

E

71–77 parent

Q

A

A

A

R

R

E

K

R

E

E

71–77 BHK5.1

Q

A

A

A

R

R

K

K

R

E

E

71–77 BHK5.2

Q

A

A

A

R

R

K

K

R

E

E

71–77 BHK10.1

Q

A

A

A

R

R

K

K

R

E

E

71–77 BHK10.2

Q

A

A

A

R

R

K

K

R

E

E

71–77 K562-VLDLR5.1

Q

A

A

A

R

R

E

K

R

E

K

71–77 K562-VLDLR5.2

Q

A

A

A

R

R

E

K

R

E

K

71–77 K562-VLDLR10.1

Q

A

A

A

R

R

E

K

R

E

K

71–77 K562-VLDLR10.2

Q

A

A

A

R

R

E

K

R

E

K

84–119 parent

Q

K

K

K

A

A

E

K

R

E

E

84–119 BHK5.1

Q

K

K

K

A

A

E

K

R

E

E

84–119 BHK5.2

Q

K

K

K

A

A

E

K

R

E

E

84–119 BHK10.1

Q

K

K

K

A

A

E

K

R

E

E

84–119 BHK10.2

Q

K

K

K

A

A

E

K

R

E

E

84–119 K562-VLDLR5.1

Q

K

K

K

A

A

E

K

R

E

E

84–119 K562-VLDLR5.2

Q

K

K

K

A

A

E

K

R

E

E

84–119 K562-VLDLR10.1

Q

K

K

K

A

A

E

K

R

K

E

84–119 K562-VLDLR10.2

Q

K

K

K

A

A

E

K

R

K

E

156–157 parent

Q

K

K

K

R

R

E

A

A

E

E

156–157 BHK5.1

K

K

K

K

R

R

E

A

A

E

E

156–157 BHK5.2

K

K

K

K

R

R

E

A

A

E

E

156–157 BHK10.1

K

K

K

K

R

R

E

A

A

E

E

156–157 BHK10.2

K

K

K

K

R

R

E

A

A

E

E

156–157 K562-VLDLR5.1

Q

K

K

K

R

R

E

A

A

K

E

156–157 K562-VLDLR5.2

Q

K

K

K

R

R

E

A

A

K

E

156–157 K562-VLDLR10.1

Q

K

K

K

R

R

E

A

A

K

E

156–157 K562-VLDLR10.2

Q

K

K

K

R

R

E

A

A

K

E

  1. Mutations that are different from the WT sequence are bolded, and mutations acquired during passage are bolded and underlined.
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