Fig. 2: Silencing tagged ABNs during memory retrieval does not impair memory. | Nature Communications

Fig. 2: Silencing tagged ABNs during memory retrieval does not impair memory.

From: Transient reactivation of small ensembles of adult-born neurons during REM sleep supports memory consolidation in mice

Fig. 2: Silencing tagged ABNs during memory retrieval does not impair memory.

a Transgenic method for tagging active ABNs in a temporally specific and reversible manner. b Visualization of Jaws-GFP in young ABNs using immunostaining, which enhanced the somatic signal (brain section from Group 3 in (c); ml, molecular layer; gl, granular cell layer; sgz, subgranular zone). Similar expression was observed in 6 mice. c Dox dose- and experience-dependent analysis of Jaws-GFP expression. d Jaws-GFP cell density (No Dox, n = 5 mice; Groups 1-2, n = 4; Group 3, n = 8). e Jaws-GFP expression in the DG in acute brain slices. The experiment was independently repeated 4 times. f–h Effect of light delivery on resting membrane potential and electrically evoked action potentials in Jaws- (n = 10) and Jaws+ (n = 4) neurons. Scale bar: 10 mV and 100 ms. Two-way repeated measures ANOVA with Sidak’s multiple comparisons. For (g), Jaws-, p = 0.99; Jaws + , p < 0.0001. For (h), Jaws-, p = 0.86; Jaws + , p < 0.0001. i, k Protocol for silencing tagged ABNs during memory retrieval. j, l Freezing during memory retrieval test. Two-tailed paired t-test. For (j), n = 14 mice, p = 0.21. For (l), n = 10, p = 0.056. TMX, tamoxifen; Dox, doxycycline; A, context A; HC, home cage; ***p < 0.001.

Back to article page