Fig. 4: Silencing ABN activity at a specific phase of the local theta oscillation in REM sleep impairs memory. | Nature Communications

Fig. 4: Silencing ABN activity at a specific phase of the local theta oscillation in REM sleep impairs memory.

From: Transient reactivation of small ensembles of adult-born neurons during REM sleep supports memory consolidation in mice

Fig. 4: Silencing ABN activity at a specific phase of the local theta oscillation in REM sleep impairs memory.The alternative text for this image may have been generated using AI.

a Transgenic method for labelling ABNs. b Halo-YFP expression in the subgranular layer of the DG. The experiment was independently repeated over 7 times/group. c Behavioural procedure for silencing ABNs in a theta phase-specific manner during REM sleep after trace fear conditioning. d Left, silencing targets within theta phases. Right, histogram of light delivery in different phase groups. 6 random REM episodes with light stimulation from individual mice LFP and the laser pulse log were analysed. Phase 1, n = 7; Phase 2, n = 9; Phase 3, n = 10; Phase 4, n = 9 mice. Dashed lines, mean phases. e Freezing in the context (left) and trace (right) tests. One-way ANOVA with Dunnett’s multiple comparisons tests (all vs. yoked control). Yoked control, n = 12 mice; Phase 1, n = 7; Phase 2, n = 9; Phase 3, n = 10; Phase 4, n = 9 mice. Context test: p = 0.042; Trace test: p = 0.013. TMX, tamoxifen; context A; B, context B; Wake, wakefulness; NREM, Non-rapid eye movement sleep; REM, REM sleep; Horizontal bars, mean; *, p < 0.05.

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