Fig. 6: Immune Cell Adhesion Assay with ICAM-1 Knockout (KO) Pluripotent Stem Cell (PSC)-Derived Endothelial Cells (ECs).

H9 wild type (WT) and ICAM-1 KO PSCs were differentiated into high-purity CD31⁺CD144⁺ ECs and stimulated with TNFα (10 ng/ml) and IFNγ (50 ng/ml) for 48 h. A U937s, B neutrophils, and C PBMCs were co-cultured with both genotypes types and washed. Each data point (n) shown in the bar graphs is representative of the total number of bound effector immune cells per regions of interest (ROI) in one experiment. The plots shown are a representative of 3 independent experiments. Images of n = 5 ROIs were acquired on ECHO Revolve | R4 microscope and analyzed using ImageJ version 1.54 g. Error bars = standard deviation. Statistical analysis via 2-tailed unpaired t-test was performed via Prism 10.2.2 software. Source data are provided as a Source Data file.