Fig. 1: Schematic of the procedure for obtaining differentially expressed genes.

A Schematic of mapping cells to determine cell types, pseudo-bulking, and obtaining cell type-specific differential expression (some cell types are removed for clarity). Orange represents AD individuals or cells, and blue represents controls. The first two sets of dots represent cells, while the third set of dots represents individuals (the sum or mean expression across all cells in a particular cell type for that individual). B Example of a gene, LINGO1, previously highlighted as upregulated in oligodendrocytes, was shown not to be upregulated in most AD datasets. Values above the line (intercept = 0, slope = 1) are upregulated, while values below the line are downregulated. Points are means, and error bars are standard deviations. Violin plots of the expression of LINGO1 in each individual across all datasets are shown in Supplementary Fig. 1. Source data are provided as a Source Data file.