Fig. 1: MV130 blocks the key pathophysiological asthma features in an experimental model of HDM-induced eosinophilic allergic airway inflammation. | Nature Communications

Fig. 1: MV130 blocks the key pathophysiological asthma features in an experimental model of HDM-induced eosinophilic allergic airway inflammation.

From: A mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway disease

Fig. 1

a Scheme of employed models. b Serum total IgE, HDM-specific IgE and IgG1 levels (PBS n = 19, MV130 n = 5, HDM n = 19, HDM + MV130 n = 18 mice; 4 independent experiments). c Left, representative hematoxylin and eosin (H&E) staining in formalin-fixed lung sections. Scale bars: 20 μm. Right, quantification of peribronchial inflammatory infiltrates in 1000 μm2 of lung sections (n = 10 bronchioles/mouse for 2 mice per group). d Quantification of total cell numbers in BALF (PBS n = 13, MV130 n = 6, HDM n = 18, HDM + MV130 n = 19 mice; 4 independent experiments). e Quantification of different cell types in BALF (PBS n = 13, MV130 n = 6, HDM n = 16, HDM + MV130 n = 17 mice; 4 independent experiments). f Left, representative α-smooth muscle actin (α-SMA) staining of formalin-fixed lung sections. Scale bars: 20 μm. Right, quantification of α-SMA staining area (μm2) relative to total bronchiole area (n = 10 bronchioles/mouse of 2-3 mice per group). g Enhanced pause (Penh) measurement at increasing doses of methacholine as determined by whole-body plethysmography (WBP)#.HDM vs MV130, *HDM vs HDM + MV130, and +HDM vs PBS comparisons (PBS n = 3, MV130 n = 4, HDM n = 4, HDM + MV130 n = 5 mice per experiment; a representative example of 2 independent experiments is shown). h Left, representative periodic acid Schiff (PAS) staining of formalin-fixed lung sections. Scale bars: 20 μm. Right, quantification of the percentage of μm2 of PAS positive staining relative to μm2 of bronchiole (n = 10 bronchioles/mouse of 2 mice per group). i Invasive lung function measurement of resistance index (RI) to increase doses of methacholine#.HDM vs MV130, *HDM vs HDM + MV130, and +HDM vs PBS comparisons (PBS n = 5, MV130 n = 4, HDM n = 5, HDM + MV130 n = 3 mice; 3 independent experiments). Values are mean ± SEM. Statistical significance was determined using unpaired One-way ANOVA (c and e (macrophages, B and T cells)), unpaired Kruskal–Wallis (b, d, e, f and h), two-side unpaired Student’s t test (e (ILC2s)) or unpaired Two-way ANOVA (g and i): *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Exact p values and source data are available in the Source Data file.

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