Fig. 2: MV130 inhibits the HDM-induced recruitment of inflammatory eosinophils (iEos) without affecting homoeostatic resident eosinophils (rEos).

a Representative flow cytometry dot plots of Siglec-F and CD125 expression on live CD45⁺ gated mouse isolated lung cells. b Histograms of CD101 (in red) and CD62L (in green) expression on inflammatory eosinophils (iEos) and resident eosinophils (rEos). Fluorescence minus one (FMO) of each fluorophore is shown in yellow. c Percentage of lung iEos and rEos relative to CD45⁺ live lung cells (n = 8 mice per group; 2 independent experiments). d Percentage of eosinophil major basic protein (MBP) positive staining in lung sections (n = 15 fields/lung/mouse for 1 mouse per group). e Representative MBP (left) and Congo Red (right) stained mouse lung sections of the peribronchial area (1 independent experiment). Scale bars: 50 μm. Black arrows point to the eosinophils. f Representative MBP (left) and Congo Red (right) stained mouse lung sections of the lung parenchyma (1 independent experiment). Scale bars: 50 μm. Black arrows point to the eosinophils. g Left, representative eosinophil peroxidase (EPX) positive stained mouse lung sections of peribronchial area. Right, percentage of EPX positive staining in lung sections (n = 13 fields/lung/mouse for 1 mouse from each group). h Left, quantification of EPX activity in BALF (PBS n = 3, HDM n = 4, HDM + MV130 n = 5 mice; 1 independent experiment). Values are mean ± SEM. Statistical significance was determined using unpaired One-way ANOVA (c, g and h) and unpaired Kruskal–Wallis test (d): *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Exact p values and source data are available in the Source Data file.