Fig. 3: MV130 restores non-pathological innate and adaptive immune responses against HDM.

Left, representative flow cytometry dot plots of intracellular staining of IFN-γ- (a), IL-5- (b) or IL-10-producing (c) lung T cells, respectively, relative to CD3⁺ lung T cells. Right, percentage of IFN-γ-, IL-5- or IL-10-producing lung T cells, respectively, relative to CD3⁺ lung T cells (n = 4 mice per group of one representative experiment out of 2). d Correlation between the percentage of iEos and the % of IL-5– (left) and IFN-γ- (right) producing lung T cells, respectively. “r” indicates Pearson correlation coefficient. e Left, representative flow cytometry dot plots of CD4⁺CD25⁺FOXP3⁺ lung Treg cells. Right, percentage of CD4⁺CD25⁺FOXP3⁺ Treg cells relative to live cells in the lungs (n = 4 mice per group of one representative experiment out of 2). f Percentage of CD23⁺ resident and migratory DCs in mediastinal lymph nodes (MLNs) (PBS n = 6, HDM n = 6, HDM + MV130 n = 10 mice; 2 independent experiments). g Percentage of total ILC2s, and IL-5-, IL-13- and IL-13/IL-5-producing ILC2s of CD45⁺ live lung cells (PBS n = 6, HDM n = 11, HDM + MV130 n = 11 mice; 2 independent experiments). h Left, levels of serum albumin in BALF 24 h after last HDM challenge (PBS n = 7, HDM n = 10, HDM + MV130 n = 11 mice; 2 independent experiments). Right, lactate dehydrogenase (LDH) levels in BALF 72 h after last HDM challenge (PBS n = 12, HDM n = 14, HDM + MV130 n = 13 mice; 2 independent experiments). Values are mean ± SEM. Statistical significance was determined using two-side unpaired Student’s t test (a), unpaired One-way ANOVA (b, e, and h), unpaired Kruskal–Wallis test (c and g), two-side Pearson test (d) and unpaired Two-way-ANOVA (f): *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Exact p values and source data are available in the Source Data file.