Fig. 6: MV130 impairs HDM-induced T2 allergic profile by generating human DCs with the capacity to polarize Th1 and IL-10 immune responses. | Nature Communications

Fig. 6: MV130 impairs HDM-induced T2 allergic profile by generating human DCs with the capacity to polarize Th1 and IL-10 immune responses.

From: A mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway disease

Fig. 6

a Scheme of the employed protocol for HmoDCs generation from healthy and HDM-allergic asthmatic donors and in vitro stimulation. b Cytokine production in cell-free supernatants after stimulation of HmoDCs with vehicle (Ctrl-), MV130, HDM or HDM/MV130 for 24 h (Ctrl- n = 17, MV130 n = 6, HDM n = 19, HDM + MV130 n = 19 healthy donors and Ctrl- n = 6, MV130 n = 6, HDM n = 6, HDM + MV130 n = 6 asthmatic donors). c Scheme of the employed protocol for HmoDCs and allogeneic naïve T cells co-cultures. d Left, cytokines produced by allogeneic naïve CD4+ T cells primed by HmoDCs in the presence of vehicle (Ctrl-), MV130, HDM or HDM/MV130 after 3 days (Ctrl- n = 9, MV130 n = 4, HDM n = 9, HDM + MV130 n = 9 healthy donors). Right, ratios of indicated cytokines were determined for HDM and HDM/MV130 stimulations (HDM n = 7, HDM + MV130 n = 7 healthy donors). e Left, cytokines produced by allogeneic naïve CD4+ T cells primed by HmoDCs in the presence of vehicle (Ctrl-), MV130, HDM or HDM/MV130 (n = 6 asthmatic donors). Right, ratios of indicated cytokines were determined for HDM and HDM/MV130 stimulations (n = 6 asthmatic donors). Values are mean ± SEM. Statistical significance was determined using two-side paired Wilcoxon or Student’s t test (b, d, e) depending on the normal distribution of the compared samples analysed by D’Agostino-Pearson normality test: *p < 0.05, **p < 0.01 and ****p < 0.0001. Exact p values and source data are available in the Source Data file.

Back to article page