Fig. 7: MV130 imprints transcriptomic and metabolic rewiring in human DCs.
a Bar plot showing gene set enrichment analysis (GSEA) the selected significantly enriched gene sets for HALLMARK, KEGG, GOBP and BIOCARTA data bases from 24 h MV130- vs vehicle (Ctrl-)-stimulated HmoDCs Gene Array (n = 4 healthy donors). Enriched gene sets in MV130-stimulated HmoDCs were represented in red and vehicle (Ctrl-)-stimulated HmoDCs were represented in blue. NES (Abs), NES absolute value. All FDR q values < 0.25. b GSEA for HALLMARK_IFN-GAMMA_RESPONSE, BIOCARTA_IL-10_PATHWAY and GOBP_FCƐR_SIGNALING_PATHWAY gene sets showing the genes enriched in the indicated biological pathways differentially enriched in MV130- or vehicle (Ctrl-)-stimulated HmoDCs (n = 4 healthy donors). c GSEA for HALLMARK_GLYCOLISIS gene set significantly enriched in MV130-stimulated HmoDCs from Gene Array results (n = 4 healthy donors). d Scheme of the employed protocol with HmoDCs for in vitro metabolic studies. e Left, real-time analysis of glycolytic Proton Efflux Rate (glycoPER) of HmoDCs after stimulation with HDM, HDM + MV130 or vehicle (Ctrl-) by sequential addition of rotenone/antimycin A (Rot/AA), and 2-deoxyglucose (2-DG). Right, quantification of basal and compensatory glycolysis (n = 4 healthy donors). f GSEA for HALLMARK_OXPHOS gene set significantly enriched in vehicle (Ctrl)-treated HmoDCs from Gene Array results (n = 4 healthy donors). g Fluorescence intensity of HDM- or HDM + MV130-stimulated HmoDCs stained with MitoTrackerTM Red CMXRos (mitochondrial membrane potential) and MitoTrackerTM Green FM (mitochondrial mass) relative to negative control-stimulated cells. MitoTracker Red/MitoTracker Green ratio is also shown (n = 10 healthy donors). Values are mean ± SEM. Statistical significance was determined using two-side paired Student’s t test (e and g): *p < 0.05, and **p < 0.01. Exact p values and source data are available in the Source Data file.
