Fig. 8: MV130 reprograming of BMDCs via MyD88-mediated pathways prevents HDM-induced eosinophilic allergic airway inflammation. | Nature Communications

Fig. 8: MV130 reprograming of BMDCs via MyD88-mediated pathways prevents HDM-induced eosinophilic allergic airway inflammation.

From: A mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway disease

Fig. 8

a Schematic representation of MV130-BMDCs or vehicle-BMDCs intranasal administration and following HDM-induced asthma model. b Serum total IgE and HDM specific IgE and IgG1 levels (PBS n = 6, Vehicle-BMDCs/HDM n = 6, MV130-BMDCs/HDM n = 7 mice; 2 independent experiments). c Quantification of total cell numbers and d of different cell types in BALF determined by flow cytometry (PBS n = 6, Vehicle-BMDCs/HDM n = 6, MV130-BMDCs/HDM n = 7 mice; 2 independent experiments). e Schematic representation of bone marrow progenitors from wild type and MyD88−/− mice differentiation to BMDCs and following in vitro stimulation with vehicle (Ctrl-), MV130, or Gefinitib/MV130. f Geometric Mean Fluorescence Intensity (GeoMFI) values for CD40 (Ctrl- n = 3-4, MV130 n = 4-5, Gefinitib/MV130 n = 5 biological replicates; 1 independent experiment). g Cytokine production in cell-free supernatants after in vitro stimulation of wild type and MyD88−/− BMDCs for 18 h (Ctrl- n = 3-4, MV130 n = 4-5, Gefinitib/MV130 n = 7 biological replicates; 1 independent experiment). h Serum total IgE and HDM specific IgG1 levels (n = 4 mice per group). i Quantification of total cell numbers and j different cell types in BALF determined by flow cytometry (n = 4 mice per group). Values are mean ± SEM. Statistical significance was determined using unpaired One-way ANOVA (b, c and i), unpaired Two-way ANOVA (f and g) and two-side unpaired Student’s t test (d and j): *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Exact p values and source data are available in the Source Data file.

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