Fig. 3: Temporal control of actin network assembly. | Nature Communications

Fig. 3: Temporal control of actin network assembly.

From: Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

Fig. 3

Representative images of the recruitment of SspB-mScarlet-I-VCA (a) and subsequent actin polymerization (b) at each indicated time point in three different illumination duration patterns. Blue squares indicate the light-illuminated regions with 1 nW/μm2. All scale bars, 20 μm. Fluorescence intensities of SspB-mScarlet-I-VCA (c) and Alexa647-actin (d), and the pillar length (e) in three different illumination duration patterns. The mean values (bold lines) are plotted as a function of time with the SD. n = 12 for each condition. Source data are provided as a Source Data file.

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