Fig. 2: Lower intranasal A. baumannii inoculums result in reduced lung neutrophil influx.
Groups of female C3H/HeN (WT) or C3H/HeJ (tlr4 mutant) mice were intranasally inoculated with 105 G636, 108 G636, or mock inoculated with PBS. At 4 h (A, D), 2 d (B, E), and 7 d (C, F) pi, alveolar macrophages (AMs) (A–C) and polymorphonuclear leukocytes (PMNs) (D–F) in the BALF were enumerated by flow cytometry. Shown are pooled results from at least two independent experiments, and each data point represents an individual mouse. The horizontal line represents the mean, and the standard error of the mean (SEM) is indicated by error bars. *p < 0.05; two-way analysis of variance (ANOVA), Tukey’s test for multiple comparisons. Shown are statistically different significances between different strains at the same inoculum and between different inoculums given to the same strain. Source data and statistical test details are provided in the Source Data file. p values: 4 hpi AMs WT, 105 vs. Mock = 0.0242; 4 hpi AMs tlr4 mutant 105 vs. 108 = 0.0003; 7 dpi AMs, WT Mock vs. 108 = 0.0272; 4 hpi PMNs, WT Mock vs. 108 = 0.0037; 4 hpi PMNs, WT 105 vs. 108 = 0.0020; 4 hpi PMNs, WT 108 vs. tlr4 mutant 108 = 0.0402; 2 dpi PMNs, WT Mock vs. 108 = < 0.0001; 2 dpi PMNs, WT 105 vs. 108 = < 0.0001; 2 dpi PMNs, tlr4 mutant Mock vs. 108 = 0.0190; 2 dpi PMNs, tlr4 mutant 105 vs. 108 = 0.0313; 7 dpi PMNs, WT Mock vs. 108 = < 0.0001; 7 dpi PMNs, WT 105 vs. 108 = < 0.0001; 7 dpi PMNs, WT 108 vs. tlr4 mutant 108 = < 0.0001.
