Fig. 2: Instrumentation of the DeMOSAIC system.
From: Optical segmentation-based compressed readout of neuronal voltage dynamics
a Schematic optical layout of the DeMOSAIC system. A motorcycle-shaped light pattern was generated by a digital micromirror device (DMD) coupled with an LED and projected onto the mirror at the sample plane (I0). The widefield image is captured by an sCMOS camera for ROI selection (I1) and is subsequently relayed to the SLMs using a motorized flip mirror (FM). Using a pair of SLMs at the conjugate image plane (I2), original image is optically segmented by introducing a distinct angular shift. The diffracted beams are refocused by a microlens array (MLA) to form 3-by-3 subimages (I3), each of which encodes the individual ROI. The subimage plane is optically demagnified and relayed to a detector (I4) in the compressed detection module. OL, objective lens. TL, tube lens. BS, beam splitter. LED, light emitting diode. M: mirror. PBS, polarizing beam splitter; HWP, half-waveplate. b A reflectance widefield image of the motorcycle-shaped pattern. Scalebar, 50 μm. c A grating pattern generated based on the selected ROIs. The 8 types of pattern units are used. d Image of the Fourier plane at MLA plane (î2), with and without pattern displayed on SLM as indicated. Scalebar, 1 mm. e The subimage plane (I3) with and without pattern displayed on SLM. Scalebar, 1 mm.
