Fig. 3: Ultrafast acquisition of a dynamic image via DeMOSAIC.
From: Optical segmentation-based compressed readout of neuronal voltage dynamics
a The experimental procedures of the DeMOSAIC acquisition equipped with a PMT array detector. Each letter in the graffiti image was selected, segmented, and assigned to each PMT channel. Scalebar, 200 μm. b Dynamic graffiti image. Using the binary pattern display mode of the DMD, each letter is displayed sequentially at a refresh rate of 9.5 kHz (105 μs for each letter with 105 or 210 μs intervals). c, d Linear unmixing of inter-channel crosstalk. Inter-channel crosstalk by −1st order signals is evident in the raw diffraction efficiency matrix and subimages (left). Linear unmixing algorithm relocates the 0th and −1st order signals to the desired 1st order (right). The dashed boxes indicate the −1st, 0th, and +1st orders for the letter ‘S’, as indicated. Scalebar, 200 μm. e The 125-kHz readout of the dynamic graffiti pattern. The data was acquired by a 9-channel PMT array at 125 kHz and post-processed by applying the linear unmixing. The sequence is decoded as ‘i AM CODES’.
