Fig. 3: Labelled RNA is detected within kidney in a small initial experiment.

a Mutation rates in SLAMseq mRNA data. Kidney RNA was alkylated and sequenced in a SLAMseq protocol designed to sequence mRNA. Increased rates of T > C conversion were detected on the positive strand; increased rates of A > G conversion were detected on the negative strand (Supplementary Fig. 5f). Box plot shows median (central line) and 1^st and 3^rd quartiles (lower and upper limits of box); whiskers define the lowest and highest values within a range extending beyond the box by 1.5x the interquartile range. b T > C conversion rates in SLAMseq mRNA data. Rates were higher in the RNA labelling group (p < 2^–16 for comparison with each of the negative control groups by Kruskal-Wallis test and post hoc Wilcoxon signed rank test). c Labelled mRNA transcripts were detected by comparing gene-wise T > C conversion rates between Cre-positive (RNA labelling) and Cre-negative (control) groups using the beta-binomial method and setting a significant false discovery rate of 0.05. d T > C conversion rates in small RNA SLAMseq data. Rates were no higher in the RNA labelling group than in the Cre -ve control group (p = 0.51 by Kruskal-Wallis test and post hoc Wilcoxon signed rank test). e Labelled RNAs in small RNA data. In contrast to the mRNA data, no small RNA labelling was detected within the kidney. Data derived from male and female mice, n = 3 in each experimental group. Source data are provided as a Source Data file.