Fig. 6: Labelled mRNA in the kidney after liver injury.

a T > C conversion rates in kidney mRNA. Rates were higher in the RNA labelling groups than the Cre -ve control group (p < 2^–16 by Kruskal-Wallis test and post hoc Wilcoxon signed rank tests). b Labelled mRNA transcripts in kidney in health. c Labelled mRNA transcripts in kidney after paracetamol. d Abundance of liver marker genes within the kidney in health. e Labelling of liver marker genes within the kidney in health (mean and bootstrapped 95% CI). f Labelling of liver marker genes in the kidney after paracetamol (mean and bootstrapped 95% CI). g T > C conversion rates in kidney small RNA. h miR-122 expression in kidney tissue: expression increased after liver injury (p = 0.0038 for comparison across all groups by Kruskal-Wallis test; p-values for post-hoc pair-wise comparisons by unpaired, two-sided Wilcoxon signed rank test are shown on the plot). Box plot shows median (central line) and 1st and 3rd quartiles (lower and upper limits of box); whiskers define the lowest and highest values within a range extending beyond the box by 1.5x the interquartile range. i Differential expression analysis of all 170 miRNAs present in kidney tissue, comparing “Labelled in health” and “Labelled in injury” groups. Differential expression was determined using a 2-group generalised linear model, adjusting for multiple comparisons using the Benjamini–Hochberg method. miR-122 was the only miRNA with significantly altered (fdr < 0.05) expression after liver injury. Data from male mice; n = 6 (labelled after paracetamol), n = 6 (labelled in health), n = 5 (Cre-negative control), n = 3 (4TU-negative control). Source data are provided as a Source Data file.