Fig. 3: PG cells undergo a major transcriptional shift between embryonic and fetal stages.
From: Defining the cell and molecular origins of the primate ovarian reserve
A UMAP plot of single cells collected from rhesus fetal ovaries at W8, W15, and W19, colored according to Seurat clusters (n = 3 biological replicates per timepoint). B Bar graph of annotated cell type proportions at each time point. C UMAP plot of W8 cells colored by cell annotation from the analysis in Fig. 3A. D Expression of ovary-enriched genes identified in the Visium CytAssist spatial transcriptomics analysis at W5 and W6 cast on the UMAP plot from Fig. 3C. Normalized expression plotted on a high-to-low scale (indigo-yellow). E UMAP plot of granulosa subset (clusters a1, a3, and a4 from 3A) re-clustered and colored according to Seurat clusters. F Bar graph of granulosa sub-cluster proportions at each time point. G Dot plot of known granulosa-associated genes, or genes identified as highly enriched in each cluster (rectangles). Expression plotted on a high-to-low scale (indigo-yellow); dot size reflects percentage of cells expressing given gene. *KRT18 = ENSMMUG00000031911), *COL9A3 = ENSMMUG00000016859.
