Fig. 3: Pupil-induced RGC responses are present across RCG types.

A UMAP of 5 clusters of RGC responses to contralateral full-field chirp stimulus (each with unique color). B Example field of view, color coded with cluster identity (representative of N = 3 animals). C Responses of RGC boutons to chirp presented to the contralateral eye, showing 3 OFF RGC and 2 ON RGC types (each with unique color as in (A)). Top row is example RGC, bottom is population average (n = 1492 boutons, N = 3 animals). D Responses of each RGC cluster in C to chirp presentation to the ipsilateral eye, color-coded as in (A). Arrowheads = responses consistent with pupil-induced responses. Box = lack of activity in amplitude or frequency ramps. E UMAP of 2 clusters of RGC ipsilateral responses to chirp stimulus in D (yellow and green). F Population responses of ipsilateral chirp responses: type 1 (middle, green) = a delayed ON response, type 2 (bottom, yellow) = a delayed OFF response followed by oscillating rebound. G Top: PLR phases during 3 s Full field flash (Supplemental Fig. 4). For boutons in each RGC subclass: peak response time (color) and suppressed response time (gray) to ipsilateral stimulation during the Full-Field Flash section of chirp stimulation for each bouton passing response threshold (OFF RGC types: 1: peak = 276, suppressed = 3, 2: peak = 137, suppressed = 1, 3: peak = 268, suppressed = 0. ON RGC types: 4: peak = 60, suppressed = 7, 5: peak = 61, suppressed = 61). Color-coded based on their contralateral response as in (A). H Same example field of view as 3B, with 2 ipsilateral clusters color-coded based on ipsi cluster obtained in (D, E). (representative of N = 3 animals). I Prevalence of each ipsilateral response in the 5 contralaterally-determined clusters, with type 1 (green) overwhelmingly in OFF RGCs and type 2 (yellow) in ON RGCs.